Figure 4.

Effect of MIP-1α secretion in IM-9 cells by intermediate of the mevalonate pathway. IM9 cells were pretreated with 1 mM MVA, 10 μM FPP, 10 μM GGPP, 30 μM squalene, 300 μM isopentenyladenine, 100 μM ubiquinone, or 60 μM dolichol for 4 h and then treated with (A) 1 μM minodronate, (B) 5 μM alendronate, (C) 0.5 μM fluvastatin, or (D) 1 μM simvastatin for 72 h. Culture supernatant was collected and analyzed for MIP-1α by ELISA. These results are representative of 5 independent experiments. *p < 0.01 vs. the controls (ANOVA with Dunnet’s test).