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. 2014 Dec 15;5(1):168–179.

Figure 6.

Figure 6

Inhibitory effect of U0126, LY294002, Y27632, or rapamycin on the secretion of MIP-1α and AML-1A mRNA expression in IM9 cells. A. Determination of the appropriate concentrations of U0126, LY294002, Y27632, or rapamycin that are not cytotoxic to IM9 cells. IM9 cells were incubated in 96-well plates for 24 h and then treated with 5 μM U0126, 1 μM LY294002, 10 μM Y27632, or 1 μM rapamycin. After 5 days, cell viability was quantified by trypan blue dye assays. The results are representative of 5 independent experiments. B. A total RNA was extracted, and MIP-1α mRNA levels were determined by real-time PCR. IM-9 cells treated with U0126, LY294002, Y27632, or rapamycin for 3 days. The results are representative of 5 independent experiments. *P < 0.01, as compared to controls (ANOVA with Dunnett’s test). C. IM-9 cells were treated with U0126, LY294002, Y27632, or rapamycin for 3 days. MIP-1α concentration of the cell culture supernatants was assayed using ELISA. The results are representative of five independent experiments. *p < 0.01 vs. the controls (ANOVA with Dunnet’s test). D. A total RNA was extracted, and AML-1A mRNA levels were determined by real-time PCR. IM-9 cells treated with U0126, LY294002, Y27632, or rapamycin for 3 days. The results are representative of 5 independent experiments. *P < 0.01, as compared to controls (ANOVA with Dunnett’s test).