Figure 2.

Effect of morusin on apoptosis in human prostate cancer cells. DU145 and M2182 cells were treated with the indicated concentration of morusin for 24 hours. A. The cells were stained with PI, and analyzed by flow cytometry. B. The cells were stained with Annexin V, and analyzed by flow cytometry. C. DU145 and M2182 cells were treated with morusin (30 μM) for 24 hours. The TUNEL and DAPI staining were analyzed by confocal microscopy. Scale bar, 70 μm. D. DU145 and M2182 cells were treated with morusin for 24 hours. Whole cell lysates were subjected to Western blotting with the indicated antibodies. β-actin was used as an internal control. Data in the graphs are presented as the mean ± SEM (*, P < 0.05; **, P < 0.01; and ***, P < 0.001 versus mock-treated control).