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. 2014 Dec 15;5(1):243–254.

Figure 2.

Figure 2

In vitro ADS-I release from ADS-I-loaded wafer and its cytotoxicity in cultured C6 cells. For each determinant, a piece of ADS-I wafer (12 mg containing 1.23 mg of ADS-I) was suspended in 7 ml of PBS containing 0.9% Peregal O (pH 7.2). At each indicated time point, the remaining ADS-I in the wafer was determined using HPLC analysis, and was used to calculate the amount of ADS-I release to the release medium. A. The percentage of ADS-I release from the ADS-I wafer. Data are represented as mean ± standard deviation (SD) of three determinants. ADS-I was released from the wafer in a time-dependent manner (p < 0.0001, one-way ANOVA, n = 3). B. The cytotoxicity of ADS-I released from ADS-I-loaded wafers was determined in C6 cell cultures using MTT assay. The same amount of ADS-I in the solution (ADS-I solution) and the same volume of the release medium from blank wafer group at each time point were used as controls. Data are represented as mean ± SD of three determinants. The cytotoxicity of released ADS-I was increased in a time/dose-dependent manner (p < 0.0001, one-way ANOVA, n = 3), and was not significantly different from that of ADS-I solution (p = 0.06, two-way ANOVA).