Figure 2.

The expression of prohibitin, MMP-2 and MMP-9 was LH-dependent. Serum-free cultures were treated with different doses of LH for 48 h (from 5 to 400 mIU/ml), preparation of protein lysates and Western blot analyses were performed as described in Materials and Methods. A. Shows the LH dose-response of prohibitin, MMP-2 and MMP-9 expression in MCV152 cells. β-actin was used as a loading control. B. Shows the LH dose-response of prohibitin, MMP-2 and MMP-9 expression in SKOV3 cells. β-actin was also used as a loading control. *represents the results of comparison with control group, which is statistically significant (P< 0.05).