Table 3.

Primers for real-time quantitative PCR

Gene name	Sequence of double-strand primers*
U6	F: 5’-TGGGGTTATACATTGTGAGAGGA-3’,
	R: 5’-GTGTGCTACGGAGTTCAGAGGTT-3’.
hsa-miR-10b	F: 5’-GGATACCCTGTAGAACCGAA-3’,
	R: 5’-CAGTGCGTGTCGTGGAGT-3’.
hsa-miR-31	F: 5’-GGAGGCAAGATGCTGGC-3’,
	R: 5’-CAGTGCGTGTCGTGGAGT-3’.
hsa-miR-205	F: 5’-GCTCCTTCATTCCACCG-3’,
	R: 5’-CAGTGCGTGTCGTGGAGT-3’.
hsa-miR-144	F: 5’-GGGGGTACAGTATAGATGAT-3’,
	R: 5’-TGCGTGTCGTGGAGTC-3’.
hsa-miR-9	F: 5’-GGGTCTTTGGTTATCTAGC-3’,
	R: 5’-TGCGTGTCGTGGAGTC-3’.
hsa-miR-202	F: 5’-GGGGAGAGGTATAGGGCA-3’,
	R: 5’-CAGTGCGTGTCGTGGAGT-3’.
hsa-let-7e	F: 5’-CAGTGCGTGTCGTGGAGT-3’,
	R: 5’-CGGTGAGGTAGGAGGTTGTAT-3’.
hsa-miR-34b	F: 5’-TGCGTGTCGTGGAGTC-3’,
	R: 5’-GCGAATCACTAACTCCACT-3’.
hsa-miR-25	F: 5’-CCAGTGCGTGTCGTGGA-3’,
	R: 5’-GGCATTGCACTTGTCTCG-3’.
hsa-miR-326	F: 5’-CCAGTGCGTGTCGTGG-3’,
	R: 5’-CCTCTGGGCCCTTCCT-3’.
hsa-miR-92a	F: 5’-ATCCAGTGCGTGTCGTG-3’,
	R: 5’-GGTATTGCACTTGTCCCG-3’.

^*F (Forward) is the corresponding miRNAs specific primer, R (Reverse) is the primer matched with RT-PCR primers.