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. 2014 Dec 15;5(1):278–288.

Figure 5.

Figure 5

Effect of curcumin on MMP-2 expression and VEGF production in HEp-2 cells. We further investigated the effect of curcumin on MMP-2 expression and VEGF production in HEp-2 cells. HEp-2 cells were treated with curcumin for 5 h. Whole-cell lysates were subjected to western-blot analysis for determining levels of total MMP-2 and VEGF proteins. A and B. Inhibition of the JAK-2/STAT-3 pathways resulted in down-regulation and suppression of MMP-2 protein. Pretreatment of HEp-2 cells with either 1 μM curcumin, 10 μM curcumin or 1 μM AG490 significantly inhibited MMP-2 protein expression. C and D. Curcumin inhibits the activation of vasculogenic endothelial growth factor (VEGF) protein expression in HEp-2 cells. Pretreatment of HEp-2 cells with 10 μM curcumin resulted in inhibition of MMP-2 and VEGF production. E and F. indicated the effect of curcumin on MMP-2 and VEGF mRNA expression in HEp-2 cells. Semi-quantitative reverse transcription-PCR of MMP-2 and VEGF in HEp-2 cells demonstrates that MMP-2 and VEGF mRNA was down-regulated expression in HEp-2 cells. GAPDH was used as an internal loading control. The data are expressed as mean ± SD of three independent experiments, and significant differences from the control are indicated by **p < 0.01.