FIG 7.

Direct binding of hexon to the Nup214(450–586) fragment. Soluble recombinant N-terminal fragments of Nup214 containing His tags were expressed and purified and coupled to nickel beads. The beads were incubated with purified hexon alone without Nup214 (lane 1), with purified hexon and Nup214(1–450) (lane 2), or with purified hexon and Nup214(450–586) (lane 3). The hexon was detected using anti-hexon antibody in the input (1/10) and unbound fractions and in the pulldown fractions. The presence of Nup214 fragments on the beads was detected using anti-V5 antibody (lower panel). IB, immunoblot.