FIG 2.

HRV replication depends on GBF1. (A) Sensitivity of HRV2 and HRV14 to GBF1 inhibitors. HeLa R19 cells were infected with virus at a low multiplicity of infection (MOI) for 30 min. After removal of the inoculum, fresh compound-containing medium was added to the cells. After 8 h, cells were freeze-thawed to release intracellular viruses. Samples were titrated on HeLa R19 cells. CVB3 was used as a positive control. Virus input levels (determined at t = 0.5 h) are indicated with a dashed line. CCID₅₀, 50% cell culture infective dose. (B) Replication rescue experiment. HeLa R19 cells were transfected with plasmids encoding EGFP, BFA-resistant GBF1-M832L, or BFA-resistant GBF1-A795E. One day later, cells were infected with virus in the presence of BFA. After 24 h, cells were freeze-thawed and samples were titrated on HeLa R19 cells.