FIG 1.

RABV P-protein constructs used in the study. P-proteins and derivatives thereof used in this study are shown schematically; constructs used to express P-proteins/derivatives from CVS RABV fused to GFP were generated by cloning into pEGFP-C1 as described previously (13) or have been described elsewhere (11). Residue positions corresponding to full-length P-protein are in italics, and regions corresponding to the N-terminal region, C-terminal domain (CTD), N-terminal NES (residues 49 to 58), and N-NLS (residues 54 to 174) are shown. The N-terminal NES mediates nuclear export of P1 but is truncated and inactivated in P3 concomitant with activation of the N-NLS. Substitution of the K and R residues at positions 54 and 55 for asparagine inhibits N-NLS function. The reported subcellular distribution of the proteins is indicated (cyt, cytoplasmic; nuc, nuclear).