FIG 4.

HSP27 phosphorylation is induced by p-MK2 upon differentiation. Cell lysates of HPV-16, HPV-18, HPV-31, and CIN 612 cells were induced to differentiate in high-calcium medium, and levels of p-HSP27 as well as total HSP-27 were examined by Western blotting. Cells were treated with 10 μM MK2 inhibitor (MK2 inhibitor III), and loss of p-HSP27 was observed. Antibodies used were anti-p-HSP-27 and anti-HSP27 (both from Cell Signaling Technologies). TG (transglutaminase) was used as a differentiation marker. β-Actin was used as a loading control.