TABLE 2.

FRET efficiencies of binary and ternary complexes between NC, dTAR, and LUVs

Monitored interactiona	Nonlabeled partner	FRET efficiency for NaCl concn:
		150 mM	30 mM
MFL-NC/LUV-Rh	−	0.84	0.90
	dTAR	0.83	0.85
dTAR-FAM/LUV-Rh	−	0	0
	NC	0.09	0.27
dTAR-FAM/LRh-NC	−	0.72	0.80
	LUVs	0.37	0.62

^aFRET experiments were performed between two labeled partners in the absence (−) and in the presence of the third nonlabeled partner in 20 mM phosphate buffer with 150 mM or 30 mM NaCl (pH 7.4).