FIG 2.

Lack of evidence for AAV ER transport. (A) HeLa cells transfected with the ER marker plasmid pER-dTmT (red) were infected with AAV2-Luc at an MOI of 10,000 and fixed at various times postinfection. Cells were immunostained with an anti-golgin-97 antibody (purple) and an anti-AAV2 capsid antibody (green). Cells were imaged by confocal microscopy. (B) Index of colocalization of AAV2 capsids with TGN or ER, expressed as the percentage of green pixels (AAV2 capsids) colocalized with TGN (purple) or ER (red) pixels. Numbers represent the mean ± SD from triplicate experiments.