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Cellular immune responses in mice. (A) A single immunization of C57BL/6 mice with 1 × 109, 1 × 108, or 1 × 107 vp of vectors expressing SIV Gag at day 0. Gag-specific CD8+ T cells were detected in serum by Db/AL11 tetramer binding assays at weekly intervals. (B) Spleens were harvested at day 28 and evaluated by IFN-γ ELISPOT assays after stimulation with the DDR13, KV9, and AL11 epitopes as well as a whole SIV Gag peptide pool. SFC, number of spot-forming cells.
