FIG 2.

Redistribution of FG-Nups in cells in which EBV was reactivated. (A) Slide-cultured NA cells were transfected with an Rta-expressing plasmid to induce EBV reactivation and harvested before induction (noninduction) and at 48 h posttransfection; the cells were then fixed and stained for BGLF4, MAb414, Nup62, Nup153, and DNA. (B) Akata cells were induced to enter the lytic cycle by IgG cross-linking and harvested at 0 and 48 h postinduction (h.p.i.). Cells were fixed and stained for BGLF4, MAb414, Nup62, Nup153, and DNA, using BGLF4 rabbit antiserum; MAb414, Nup62, and Nup153 antibodies; and Hoechst 33258, respectively. (C) TW01/p2089 (WT) or TW01/p2089BGLF4stop (BGST 52-1-2) pooled clones were cotransfected with plasmids expressing Rta and Zta (R+Z) or the vector control to induce EBV lytic replication and harvested at 48 h posttransfection. Cells were fixed and stained for MAb414. The distribution of BGLF4 and nucleoporins was observed by confocal microscopy.