FIG 1.

VHSV replication in trout spleen IgM⁺ cells. (A) Transcription of the VHSV N gene was assessed by real-time PCR in sorted IgM⁺ cells after 24 h of viral inoculation. Means of data from 6 independent experiments are shown. (B) VHSV N protein expression in IgM⁺ and IgM⁻ sorted cells previously incubated with the virus during 24 h was evaluated by Western blotting. β-Actin was included as a loading control.