FIG 5.

Intercellular connections form in paramyxovirus-infected cells and contain the RNP components N and P. A549, MDCK, and Vero cells were infected with PIV5 at an MOI of 3 and fixed at 24 h p.i. Cell surfaces were immunostained for the fusion protein (F; green) and then permeabilized and immunostained for either nucleoprotein (N, red) (A) or P protein (red) (B). Infected cells formed intercellular connections between neighboring cells that appeared to connect the cytoplasm of both cells. Images were photographed on a confocal microscope. Scale bar, 20 μm.