FIG 7.

Intercellular connections form in IAV-infected primary cells. Normal human bronchial epithelial cells (NHBE) were infected with IAV (MOI = 1) or mock infected and fixed at 17 h p.i. in 10% formalin. IAV-infected cell surfaces were immunostained for hemagglutinin (HA; green), and then the cells were permeabilized and immunostained for polymerase (PA; red). Mock-infected cells were stained with Alexa Fluor 488-conjugated wheat germ agglutinin (WGA) to visualize the cell surface. Images were photographed on a confocal microscope. Scale bar, 20 μm.