FIG 2.

Protein compositions of HSV-1 WT and mutant virus capsids as determined by Western blot analysis of cytoplasmic C-capsids purified from the cytoplasm of cells infected with WT HSV-1 or the inner tegument deletion mutants ARΔUL36 and FRΔUL37. Serial 2-fold dilutions of each sample were run on a 10% polyacrylamide gel and transferred to Hybond ECL nitrocellulose membranes for analysis. Equal amounts of purified WT virions were loaded for comparative purposes. Proteins were visualized using antibodies MAbE12-E3 (pUL36), M780 (pUL37), MAb203 (pUL17), and MAb166 (pUL25). The major capsid protein pUL19 was monitored as a loading control using antibody DM165. The positions of molecular weight standards (in thousands) are indicated to the right of each panel.