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. Author manuscript; available in PMC: 2015 Jan 21.
Published in final edited form as: Nat Struct Mol Biol. 2010 Dec 26;18(1):6–13. doi: 10.1038/nsmb.1979

Figure 4.

Figure 4

Localization of Doc1 and Swm1 in the yeast APC/C 3D structure by subunit deletion and td2–labeling. (a) 3D models illustrating Doc1 localization. Density not present in APC/CΔDoc1 compared to APC/CWT is labeled as main density difference in the APC/C WT – ΔDoc1 difference map. Subunit deletion and td2–labeling converge at identical locations. (b) Doc1–td2–labeling and EM analysis compared to wild type APC/C. Class averages of wild type and Doc1–td2–labeled APC/C are shown in two different orientations. The additional density caused by the td2–tag is indicated with arrows in the class averages. 3D models computed from the corresponding datasets are depicted in similar orientations. (c) Localization of Swm1 using subunit deletion and td2–labeling. Swm1 is located in the head domain. The size bars illustrate a distance of 10 nm. The asterisks mark the face of APC/C’s central cavity.