Table 4.
The validation of bra-miR168a-1 and braAGO1 expression in tissues of heat-shock-stressed B. rapa parental plants and untreated progeny.
| Tissue | log2FC TR vs. CT, bra-miR168a-1 expression, sequencing | q-value, sequencing | Normalized log2FC TR vs. CT, bra-miR168a-1 expression, Northern Blot | log2FC TR vs. CT braAGO1 expression, sequencing | q-value, sequencing | log2FC TR vs. CT braAGO1 expression, qPCR | p-value, TR vs. CT, qPCR |
|---|---|---|---|---|---|---|---|
| Leaves | 0.58 | 1 | 3.15 | −0.08 | 1 | 0.74* | 2.13E-02 |
| Inflorescence meristem | 0.05 | 1 | −0.07 | 0.41 | 1 | 0.69* | 6.00E-04 |
| Pollen | 1.19* | 0.04 | 0.36 | 0.64 | 1 | 1.08* | 2.01E-04 |
| Unfertilized ovules | −0.54 | 1 | −0.21 | −0.08 | 1 | −0.81* | 5.04E-03 |
| Fertilized ovules | −0.62 | 1 | 0.64 | −0.04 | 1 | 0.70* | 1.26E-04 |
| Embryo | 0.61 | 1 | 2.50 | −0.77* | 1.29E-02 | −0.60* | 4.12E-03 |
| Endosperm | 3.42* | 0.41 | 1.16 | −1.56* | 1.36E-04 | −0.81* | 1.23E-03 |
| Progeny | −0.58* | 0.93 | −1.12 | 0.06 | 1 | 0.57* | 2.03E-02 |
| Correlation, braAGO1 expression vs. bra-miR168a-1, sequencing data | Pearson's r = −0.66 | ||||||
The asterisks denote a significant difference in the expression as compared to controls (the Benjamini-Hochberg method, q < 0.2 and the Student's t-test, P < 0.05 for sequencing and qPCR data, respectively). Log2FC—estimated log2 value of fold change for treated plants vs. controls.