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Example of results obtained with the amoeba predation assay. Various quantities of amoebae were deposited on P. aeruginosa lawns (5 to 50,000 amoebae per spot). After a 7-day incubation, phagocytic plaques were visible where amoebae fed on the bacteria. The growth of the amoebae fed on the lawns of two isolates is illustrated: (A) Isolate 578-B is sensitive to predation; (B) Isolate PPF 2 is resistant to predation. The negative control (Ctrl-) was HL5 medium without amoeba.
