Table 1.
Bacterial strains and plasmids.
| Strains or plasmids | Relevant characteristics | References |
|---|---|---|
| E. COLI | ||
| DH5α (PAGUg121) | For recombinant DNA manipulation | Morita et al., 2010 |
| KAM3 (PAGUg846) | For recombinant DNA manipulation | Morita et al., 1998 |
| S17-1 (PAGUg856) | For conjugational transfer | Morita et al., 2006 |
| P. AERUGINOSA | ||
| PAO1 (PAGU 974) | Wild type | Morita et al., 2006 |
| PA7 (PAGU 1498) | Multi-resistant clinical isolate | Roy et al., 2010 |
| PAGUg1565 | PA7 ΔmexXY-oprA | Morita et al., 2012b |
| PAGUg1603 | PA7 ΔmexAB-oprM | Morita et al., 2012b |
| PAGUg1748 | PA7 ΔmexEF-oprN | This study |
| PAGUg1641 | PA7 ΔmexXY-oprA ΔmexAB-oprM | Morita et al., 2012b |
| PAGUg1751 | PA7 ΔmexXY-oprA ΔmexEF-oprN | This study |
| PAGUg1753 | PA7 ΔmexAB-oprM ΔmexEF-oprN | This study |
| PAGUg1756 | PA7 ΔmexXY-oprA ΔmexAB-oprM ΔmexEF-oprN | This study |
| PAGUg1793 | PA7 ΔmexS | This study |
| PAGUg1789 | PA7 ΔmexT | This study |
| PAGUg1867 | PA7 mexS(V155A) | This study |
| DSM 1128 (PAGU 1504) | Taxonomically PA7-related strain, wild-type | Kiewitz and Tummler, 2000 |
| K2153 (PAGU 1741) | Clinical isolate | Sobel et al., 2003 |
| K2376 (PAGUg1834) | K2153 ΔmexS | Fetar et al., 2011 |
| PAGUg1850 | K2153 ΔmexS attB::lacIq-PT7 | This study |
| PAGUg1851 | K2153 ΔmexS attB::lacIq-PT7-mexSPA7 | This study |
| PAGUg1844 | K2153 ΔmexS attB::lacIq-PT7-mexSDSM 1128 | This study |
| PAGUg1854 | K2153 ΔmexS pUCP20T | This study |
| PAGUg1855 | K2153 ΔmexS pUCP20T::mexSPA7 | This study |
| PAGUg1856 | K2153 ΔmexS pUCP20T::mexSDSM 1128 | This study |
| PAGUg1835 | K2153 ΔmexT | Fetar et al., 2011 |
| PAGUg1836 | K2153 ΔmexF | Fetar et al., 2011 |
| K2942 (PAGUg1837) | K2153 ΔmexS ΔmexT | Fetar et al., 2011 |
| PAGUg1852 | K2153 ΔmexS ΔmexT attB::lacIq-PT7 | This study |
| PAGUg1846 | K2153 ΔmexS ΔmexT attB::lacIq-PT7-mexTPA7 | This study |
| PAGUg1845 | K2153 ΔmexS ΔmexT attB::lacIq-PT7-mexTDSM 1128 | This study |
| PLASMID | ||
| pEX18Tc | Broad-host-range gene replacement vector | Hoang et al., 1998 |
| pYM133 | pEX18Tc::ΔmexEF-oprN | This study |
| pYM134 | pEX18Tc::ΔmexS | This study |
| pYM135 | pEX18Tc::ΔmexT | This study |
| pYM136 | pEX18Tc::ΔmexST | This study |
| pYM137 | pEX18Tc::mexSPA7 | This study |
| pYM138 | pEX18Tc::mexSPA7(V155A) | This study |
| pYM101 | P. aeruginosa chromosome integration vector | Morita et al., 2010 |
| pYM139 | pYM101::mexSPA7 | This study |
| pYM140 | pYM101::mexSDSM 1128 | This study |
| pYM141 | pYM101::mexTPA7 | This study |
| pYM142 | pYM101::mexTDSM 1128 | This study |
| pUCP20T | Broad-host-range cloning vector | Schweizer et al., 1996 |
| pYM143 | pUCP20T::mexSPA7 | This study |
| pYM144 | pUCP20T::mexSDSM 1128 | This study |
| pFLP2 | Flp recombinase-producing plasmid | Hoang et al., 1998 |