Table 5.

Functional characterization of mexS and mexT from P. aeruginosa PA7 and DSM 1128.

Strains	Genotype	MIC (μg/ml)
		CIP	CHL	IPM
K2153 (PAGU 1741)	Parent	0.5	64	2
K2376 (PAGUg1834)	ΔmexS	2	2048	8
PAGUg1850	ΔmexS attB::lacIq-PT7	2	2048	8
PAGUg1851	ΔmexS attB::lacIq-PT7-mexSPA7	2	2048	8
PAGUg1844	ΔmexS attB::lacIq-PT7-mexSDSM 1128	2	1024	8
PAGUg1854	ΔmexS pUCP20T	2	2048	8
PAGUg1855	ΔmexS pUCP20T::mexSPA7	1	256	4
PAGUg1856	ΔmexS pUCP20T::mexSDSM 1128	0.5	64	2
K2942 (PAGUg1837)	DmexS ΔmexT	0.5	64	2
PAGUg1852	ΔmexS ΔmexT attB::lacIq-PT7	0.5	64	2
PAGUg1846	ΔmexS ΔmexT attB::lacIq-PT7-mexTPA7	2	2048	8
PAGUg1845	ΔmexS ΔmexT attB::lacIq-PT7-mexTDSM 1128	2	2048	8

CHL, chloramphenicol; CIP, ciprofloxacin; IPM, imipenem.

5 mM IPTG was added to allow expression controlled by the P_T7 promoter.