Table 2.
“In silico” characterization of the COI restriction sites using single enzyme reactions with AluI and MboI or a double digest reaction with AseI/TaqI in nine species of whiteflies
| WF1 species | AluI | MboI | AseI + TaqI | |||
|---|---|---|---|---|---|---|
| No. frag.(s) | Length(s) | No. frag.(s) | Length(s) | No. frag.(s) | Length(s) | |
| Aleurodicus dispersus | 5 | 336, 174, 118, 69 & 12 | 2–4 | 6152, 611, 98, & 94 | 5–7 | 207, 172, 151, 95, 84 & 122 |
| Aleuronudus melzeri | 4 | 330, 243, 124 & 12 | 7 | 328, 128, 78, 77, 53, 27 & 18 | 6 | 202, 172, 119, 95, 89 & 32 |
| Aleurothrixus floccosus | 3–5 | Undigested (709)2, 591 5362, 118 & 552 | 6 | 294, 128, 112, 104, 53 & 18 | 2 | 649 & 60 |
| Aleurotrachelus socialis | 3–6 | 6112, 454, 3302, 157 & 98 | 3–5 | 6152, 611, 53, 492 & 45 | 3 | 554, 95 & 60 |
| Aleurotrachelus trachoides | 2–3 | Undigested (709)2, 575 & 134 | 4 | 534, 77, 53 & 45 | 3 | 391, 263 & 55 |
| Bemisia tabaci (A)3 | 3–7 | 5912, 4542, 336, 255, 118, 822, & 36 | 5 | 483, 128, 71, 16 & 11 | 5 | 483, 95, 60, 50 & 21 |
| Bemisia tabaci (B)3 | 4 | 367, 157, 98 & 87 | 6 | 534, 77, 53, 18, 16 & 11 | 5 | 354, 129, 95, 71 & 60 |
| Lecanoideus floccissimus | 5–7 | 5222, 4542, 336, 2432, 174, 118, 69 & 12 | 6–11 | 4102, 406, 1322, 128, 812, 77, 53, 312, 27 222& 18 | 8 | 241, 133, 122, 95, 84, 21, 8 & 5 |
| Trialeurodes vaporiariorum | 1 | Undigested (709) | 5 | 456, 78, 77, 53 & 45 | 4 | 303, 174, 143 & 89 |
| Trialeurodes variabilis | 5–7 | 3222, 2552, 191, 134, 132, 131,121 & 47 | 6 | 212, 199, 134, 71, 66 & 27 | 5 | 255, 169, 130, 95 & 60 |
1
Whitefly.
2
Intraspecific variation explaining WF population differences.
3
Samples associated with Phaseolus vulgaris.