Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 30;10:41. doi: 10.1186/s13007-014-0041-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Etherington, Monaghan et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Pipeline for bulking segregants and identification of unique SNPs. (A) The recessive bak1-5 mob1 and bak1-5 mob2 mutants were back-crossed to the parent bak1-5, allowed to self-fertilize in the F₁, and phenotypically scored in the F₂ for the mob phenotype. Positive segregants were bulk harvested and genomic DNA was prepared and sequenced using the Illumina HiSeq platform. For comparison, the bak1-5 genome was also sequenced. A similar genetics pipeline could be employed for dominant mutants, but material would need to be bulked from segregants that were phenotypically verified as homozygous in the F₃ generation. (B) A three-way comparison between the bak1-5, bak1-5 mob1, and bak1-5 mob2 genomes identified the total number of unique SNPs in each genome.