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. 2014 Dec 23;7:102. doi: 10.1186/s13039-014-0102-7

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© Trofimova et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sensitivity of G-rich PO41 transcripts during the cell cycle to RNases with different substrate specificity. Treatment of MDCC-MSB1 cells with different RNases before or after (RNase H*) DNA/RNA FISH with PO41neg (red) probes. MDCC-MSB1 cells are shown at interphase, prophase, metaphase, anaphase and telophase stages. RNases used for treatments are indicated on the left. PO41 RNA was degraded by RiboShredder RNases cocktail, RNase A treatments and RNase H treatment performed after FISH. RNase H and RNase III treatments completely removed only dispersed G-rich transcripts at interphase and decreased the intensity of RNA FISH signals at other stages of cell cycle. DNA was counterstained with DAPI. Scale bar: 5 μm.