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. 2014 Dec 23;7:102. doi: 10.1186/s13039-014-0102-7

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© Trofimova et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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C-rich transcripts of PO41 repeat in chicken small intestine cells. FISH with PO41pos (green, upper row) and PO41neg (red, bottom row) probes on small intestine cryosections. (a, a’) DNA/RNA hybridization revealed C-rich (green), but not G-rich (red) PO41 repeat transcripts in mucous membrane of chicken small intestine. (b, b’) DNA/DNA hybridization (positive control) revealed clusters of PO41 repeat in all cell nuclei. (c, c’) RNase A treatment before DNA/RNA hybridization (negative control) removed hybridization signals with PO41pos probe from the cell nuclei, but didn’t remove signals in cytoplasmic granules within the mucous membrane. Right-hand images demonstrate enlarged fragments of muscles layer (upper panels) and mucous membrane (bottom panels) of small intestine. Nuclei were counterstained with DAPI. Scale bar: 40 μm.