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Design of class-selective histone demethylase probe for affinity pulldown. (A) Classification of histone lysine demethylases. (B) Structures of bivalent demethylase inhibitor, methylstat acid (1), and its methyl ester, methylstat (2). (C) Structures of methyllysine-αKG mimic conjugates.(D) Structure of a highly functionalized H3K36me2-JHDM-targeting peptidic probe for affinity pulldown.
