Table 1. Epigenetic Proteins Specifically Pulled down by Peptidic Probe 6a.
| Uniprot accession number | gene name | epigenetic protein family | endogenousb | negativec | spiked-ind |
|---|---|---|---|---|---|
| Q15652 | JMJD1C/KDM3C | histone lysine demethylase | 6 | 0 | 3 |
| O75164 | JMJD2A/KDM4A | histone lysine demethylase | 0 | 0 | 5 |
| Q9UPP1 | PHF8/KDM7A | histone lysine demethylase | 1 | 0 | 1 |
| Q9HCK8 | CHD8 | chromodomain-containing protein | 2 | 0 | 3 |
| Q9P2D1 | CHD7 | chromodomain-containing protein | 5 | 0 | 1 |
| Q13547/Q92769 | HDAC1/2 | histone lysine deacetylase | 8 | 0 | 3 |
a
The spectral count is listed for three pulldown experiments.
b
The endogenous experiment corresponded to the addition of the peptidic probe 6.
c
The negative control corresponded to the absence of peptidic probe 6.
d
The spiked-in experiment corresponded to endogenous conditions with the addition of recombinant KDM4A to the nuclear extract.