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RPA is required for RNA rolling-circle synthesis. (A and B) Schematic representations of the templates that were used in the reactions. (C and D) NS5BΔ21 (open squares) or NS5BΔ21-NS3h (filled squares) were assembled onto a rolling-circle template without RPA (C) or in the presence of RPA (D). Synthesis was initiated by adding NTP, aliquots were withdrawn at the indicated time points, and [32P]UMP incorporation was determined as described in Materials and Methods.
