FIG 3.

RPA and gp32 interact with RNA. (A) ³²P-RNA gel shift assay. ³²P-RNA was preincubated with RPA or gp32 and loaded onto a 10% agarose gel. The two proteins interact with the RNA with similar affinities, producing a supershifted band. (B1) NS5BΔ21 synthesis on a poly(A)/oligo(dT) template: The reaction was started by the addition of NTP, and the incorporation [³²P]UTP into the synthesized product was measured in samples obtained at the indicated time points. (B2) NS5BΔ21 synthesis in the presence of RPA on the poly(A)/oligo(dT) template. Conditions were as described above. (B3) NS5BΔ21 synthesis in the presence of gp32 on the poly(A)/oligo(dT) template. Conditions were as described above.