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. 2014 Dec 16;89(1):165–180. doi: 10.1128/JVI.01677-14

FIG 5.

FIG 5

RPA ties NS5BΔ21 to the template. 35S-labeled NS5BΔ21 and NS3h, individually synthesized in transcription/translation reactions in vitro in the presence of [35S]methionine, were incubated with circular ssM13mp18 DNA coated with RPA (A and D) or gp32 (B and E) or alone (C and F) and run on a BioGel A100 5-ml gel filtration column to resolve protein bound to DNA (fractions 10 to 15) from free protein (fractions 16 to 30). 35S-NS5BΔ21 stably interacted with ssDNA coated with RPA but not with ssDNA alone or ssDNA coated with T4 gp32. 35S-NS3h, on the other hand, did not show a stable interaction with ssDNA alone or when coated with RPA or T4 gp32.