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. 2014 Dec 16;89(1):165–180. doi: 10.1128/JVI.01677-14

FIG 7.

FIG 7

Preformed NS3h-NS5BΔ21 complex is a stable functional complex. (A) 35S-NS3h–NS5BΔ21 complex formation and isolation. Superose 12 gel filtration chromatography profiles of 35S-NS3h alone (open squares) and with preincubation with cold NS5BΔ21 (filled diamonds). The gray box shows fractions 32 to 38 of 35S-NS3h–NS5BΔ21 complex, which were pooled and examined for interaction. (B) 35S-NS3h–NS5BΔ21 complex interaction with ssDNA (filled diamonds) and with ssDNA coated with RPA (open squares) on a BioGel A100 gel filtration column.