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. 2014 Dec 16;89(1):165–180. doi: 10.1128/JVI.01677-14

FIG 9.

FIG 9

RPA and NS5A cooperate to form RNA polymerase processivity factor. The radiolabeled RNA products resulting from in vitro reactions carried out as described in Materials and Methods were analyzed by polyacrylamide gel electrophoresis and autoradiographed. (A) RNA synthesis by NS5BΔ21-NS3h from the rolling-circle template in the presence of RPA without and with poly(A)/oligo(U). (B) RNA synthesis by NS5BΔ21-NS3h from the rolling-circle template in the presence of NS5A(S25-C447) without and with poly(A)/oligo(U). (C) RNA synthesis by NS5BΔ21-NS3h from the rolling-circle template in the presence of RPA and NS5A(S25-C447), without and with poly(A)/oligo(U).