FIG 5.
Transcriptional profile of wild-type BAC16 and ORF45 mutants in iSLK cells. Stable iSLK cells carrying wild-type BAC16, F66A, F66A-rev, or Stop45 viral genomes were induced with doxycycline and sodium butyrate for 48 h. Total RNA was extracted, reverse-transcribed into cDNA, and used for KSHV whole-genome qPCR array analysis. The delta CT values for each primer set were calculated and converted to a heat map using R. ORFK1 was omitted because the primers failed to amplify significant product. IE, immediate early; E, early; L, late; −, unknown expression kinetics.