FIG 4.

Analysis of the effects of the 5Mut substitutions on PF74 resistance and PF74 binding to virions. (A) Quantification of IC₅₀ values for viral inhibition. Shown are the mean values, determined from three or four independent experiments, depending on the mutant. Error bars represent 1 standard deviation. (B) Extent of binding of PF74 to virions. Concentrated virus particles were incubated with [³H]PF74 at room temperature and pelleted by ultracentrifugation. Pellets were assayed for ³H by scintillation counting and for viral antigen by p24 ELISA. The values shown represent the ratio of ³H to p24, normalized to wild-type HIV-1. Shown are the mean values from three independent determinations, with error bars representing 1 standard deviation.