FIG 5.
Conferring DCAF1 interaction to SIVdeb Vpr does not lead to G2/M arrest or human SLX4 complex interaction. (A) Alignment of SIVdeb and SIVmus and chimeric Vpr proteins. (B) DNA content analysis of HeLa cells transiently transfected with the constructs as described for panel A and Fig. 1. (C) Quantification of G2:G1 ratios of the data presented in panel B. Columns and error bars represent means ± standard deviations of the results of 3 independent experiments. (D) 293T cells transfected with FLAG-tagged SIVdeb, SIVmus, or chimeric Vpr expression vectors in combination with HA-DCAF1. At 48 h later, anti-FLAG immunoprecipitates and cell lysates were subjected to Western blotting for anti-HA and anti-FLAG. (E) The immunoprecipitates and lysates described in the panel D legend were further analyzed for endogenous SLX4 and MUS81 coprecipitation.