TABLE 3.

Sequence analysis of progeny HSV-1 genomes in the brains of mice infected with YK751 (vdUTPaseS187A) or YK752 (vdUTPaseS187A-repair)

Virus	No. of expt	Total no. of clones analyzed	No. of mutated clones	Frequency of mutated clonesa	Total no. of nucleotides analyzed	No. of mutated nucleotides	Mutation frequencyb	Fold enhancementc
vdUTPaseS187A	1	74	9		47,582	9	18.9 × 10−5
	2	93	14		59,799	14	23.4 × 10−5
	Mean	83.5	11.5	0.138	53,691	11.5	21.2 × 10−5	3.79
vdUTPaseS187A-repair	1	88	2		56,584	2	3.54 × 10−5
	2	74	5		47,582	5	10.5 × 10−5
	Mean	81	3.5	0.0432d	52,083	3.5	7.02 × 10−5	1.00

^aFrequency of mutated clones was calculated as (mean of number of mutated clones)/(mean of total number of clones analyzed).

^bMutation frequency was calculated as (number of mutated nucleotides)/(total number of nucleotides analyzed).

^cFold enhancement was calculated as (mean of mutation frequency of vdUTPaseS187A genome) or (mean of mutation frequency of vdUTPaseS187A-repair genome)/(mean of mutation frequency of vdUTPaseS187A-repair genome).

^dStatistically significant difference from vdUTPaseS187A (P < 0.001).