TABLE 1.
Summary of HCMV BACs used in this study
| Construct | Genetic background | Reference for background construct | Change(s) introduced |
|---|---|---|---|
| WT LMN-ΔH/C | WT_E7 AD169-RV | Kamil et al. (16) | Sequence encoding the dominant negative GFP-lamin A mutant, lacking the lamin A head and CaaX domains, cloned downstream of a duplicated UL97 promoter region in the US9-US10 intergenic region |
| Δ97 LMN-ΔH/C | AD169-RV | Hobom et al. (32) | Sequence encoding the dominant negative GFP-lamin A mutant, lacking the lamin A head and CaaX domains, cloned to replace the UL97-encoding sequence. |
| S216A | 53-F AD169-RV | Sharma et al. (8) | Alanine replacement of UL50 serine-216 in UL53-FLAG genetic background |
| S19A | 53-F AD169-RV | Sharma et al. (8) | Alanine replacement of UL53 serine-19 in UL53-FLAG genetic background |
| S216A/S19A (AA) | 53-F AD169-RV | Sharma et al. (8) | Double alanine mutant carrying alanine replacements of UL50 serine-216 and UL53 serine-19 in UL53-FLAG genetic background |
| S216AR/S19AR (RR) | AA-53-F AD169-RV | This study | Rescued derivative of alanine double mutant described above, restoring WT serine-encoding sequences to UL53 and UL50 |
| S216E/S19E (EE) | 53-F AD169-RV | Sharma et al. (8) | Double mutant carrying glutamate replacements of UL50 serine-216 and UL53 serine-19 in UL53-FLAG genetic background |
| K355Q 53-F | 53-F AD169-RV | Sharma et al. (8) | UL97 kinase active site mutation (mutation of lysine 355 to glutamine) in UL53-FLAG AD169rv genetic background |