TABLE 3.
Phosphopeptides detected in MS analysis of the in vitro kinase assay with GST-UL97 and UL53(1–292)
| Residue(s) | Phosphorylated peptide detected by MSa | % of peptide phosphorylatedb |
|
|---|---|---|---|
| −MBV | +MBV | ||
| S19 | SALRSpLLR | 100 | NDd |
| S15/S19 | SpALRSpLLR | 100 | ND |
| S31 | QRELASpK | 11.7 | 0.7 |
| S35/T36 and T41/S42c | VASpTpVNGATpSpANNHGEPPSPADARPR | 4.1 | ND |
a
Sp, phosphorylated serine; Tp, phosphorylated threonine.
b
The percentage of phosphorylated peptide versus nonphosphorylated peptide was determined from peak intensities.
c
The phosphorylated serine/threonine residue could not be positively localized.
d
ND, phosphorylated peptide was not detected.