TABLE 1.
Frequency of WHVNY RNA detection in nonmalignant liver tissues and HCC samples
| Woodchuck and tissue typea | Tissue sampleb | Frequency of WHVNY RNA detectionc |
|---|---|---|
| F7808 | ||
| Liver | LM | 1/7 |
| RL | 2/8 | |
| LL | 1/11 | |
| HCC | T1 | 1/7 |
| T2 | 3/10 | |
| T3 | 2/5 | |
| F7806 | ||
| Liver | LM | 2/2 |
| RL | 2/3 | |
| LL | 3/6 | |
| HCC | T1 | 2/6 |
| T2 | 3/4 | |
| T3 | 3/3 | |
| M7761 | ||
| Liver | LM | 3/3 |
| RL | 4/4 | |
| LL | 2/2 | |
| HCC | T1 | 3/5 |
| T2 | 2/2 | |
| T3 | 2/2 | |
| T4 | 3/3 | |
| T5 | 3/3 |
The current study used three WHV7 carrier woodchucks, F7808, F7806, and M7761, which were superinfected with another WHV strain, WHVNY.
The analyzed tissue samples were harvested during necropsy at 6 weeks after superinfection with WHVNY. For each animal, the nonmalignant liver tissues from left medial liver lobe (LM), right lateral liver lobe (RL), and left liver lobe (LL) were collected. In addition, the tissues samples (the center cores) also were harvested from each of the WHV-induced HCCs that were identified in the livers during necropsy. T1 stands for HCC1, T2 for HCC2, etc.
For each tissue sample, two or more independent isolations of the total RNA and the subsequent analysis for the presence of WHVNY RNA were conducted. The numbers in the table reflect the number of occasions during which WHVNY RNA was detected using a conventional seminested PCR assay relative to the total number of independent RNA isolations performed.