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Individuals were tested for the presence of anti-MCMV antibodies by ELISA (A) and for the presence of MCMV DNA by nested PCR (B). Filled circles, positive individuals; open circles, negative individuals. Distances are given in kilometers. The gray line represents the Czech-German border, and the dashed line marks the consensus hybrid zone center as estimated by Macholán et al. in 2008 (51).
