FIG 7.

MAV-1 persistence and cardiac hypertrophy. Mice were infected with MAV-1 and hearts harvested at 9 weeks postinfection. (A) Nested PCR was used to evaluate the presence of MAV-1 DNA (top) and β-actin (bottom). (B) Left ventricular mass (LV) was measured by echocardiography and normalized to tibia length (TL). (C) Heart sections were stained with FITC-conjugated wheat germ agglutinin to outline cell borders. The cardiomyocyte cross-sectional area was measured from digital images using NIH ImageJ software. Combined data from >350 cells for each condition are presented as means ± SEM. ***, P < 0.001; **, P < 0.01 (comparing mock to MAV-1 infection).