TABLE 1.
Primers and probes used for real-time PCR analysis
| Target | Oligonucleotide | Sequence (5′ to 3′) |
|---|---|---|
| MAV-1 E1A genomic | Forward primer | GCACTCCATGGCAGGATTCT |
| Reverse primer | GGTCGAAGCAGACGGTTCTTC | |
| Probe | TACTGCCACTTCTGC | |
| GAPDH | Forward primer | TGCACCACCAACTGCTTAG |
| Reverse primer | GGATGCAGGGATGATGTTC | |
| IFN-γ | Forward primer | AAAGAGATAATCTGGCTCTGC |
| Reverse primer | GCTCTGAGACAATGAACGCT | |
| TNF-α | Forward primer | CCACCACGCTCTTCTGTCTAC |
| Reverse primer | AGGGTCTGGGCCATAGAACT | |
| MAV-1 E1A | Forward primer | AATGGGTTTTGCAGTCTGTGTTAC |
| Reverse primer | CGCCTGAGGCAGCAGATC | |
| MAV-1 hexon | Forward primer | GGCCAACACTACCGACACTT |
| Reverse primer | TTTTGTCCTGTGGCATTTGA |