FIG 1.

Alphavirus multiplication is restricted in differentiated AP-7 neurons. cAP-7 (dashed lines) or dAP-7 (solid lines) neurons were infected with the TE strain of SINV (MOI, 5) (A) or TC-83 strain of VEEV (MOI, 50) (B). Viral titers in supernatant fluids collected at the indicated times after infection were measured by plaque formation in BHK cells and are expressed as the mean log₁₀ PFU/ml ± standard deviation of triplicate samples from a representative of three experiments. (C) Percentages of cAP-7 and dAP-7 neurons expressing detectable E2 glycoprotein at the indicated times after infection were determined by immunofluorescence. E2-positive cells compared to the total number of DAPI-positive cells were determined in three nonoverlapping fields.