FIG 8.

Medium exchange does not confer antiviral properties. cAP-7 neurons were incubated in normal medium (DMEM–10% FBS), differentiation medium, or supernatant fluids from dAP-7 neurons for 24 h. (A) Extracellular virus collected at 5 and 24 h after SINV infection (MOI, 5) infection was measured by plaque assay. Titers are expressed as the mean log₁₀ PFU/ml ± standard deviation of triplicate samples. (B) Total cellular RNA was collected, and cDNA was produced using random primers. Gene expression was measured by qPCR and normalized to glyceraldehyde-3-phosphate dehydrogenase levels. RNA levels are expressed as the mean value compared to levels in cAP-7 neurons in normal medium ± standard deviation of six samples. Significant differences by Student's t test or one-way ANOVA with Dunnett's posttest are shown. **, P < 0.01.