FIG 4.

The actomyosin system is involved in NB motility. (A to C) Confocal imaging of NBs and actin filaments. NP:CFP and YFP:FABD2 (pseudocolored magenta) were coexpressed in N. benthamiana leaves and observed at 36 hpi. (A) Three-dimensional projection of entire z-stacks. NP:CFP localized along actin filaments. (B) A magnified image of the experiment shown in panel A. (C) Integration of time-lapse images of NP:CFP. (D) YFP:FABD2 (pseudocolored cyan), NP:CFP (pseudocolored yellow), and mCherry:HDEL were coexpressed and observed at 36 hpi. (E and F) Effects of inhibition of actin filaments on the motilities of the Golgi apparatus and NBs. Leaves expressing ManI:CFP (E) or NP:YFP (F) at 26 hpi were treated with 0.5% DMSO (left column) or 10 μM LatB (right column) and observed at 36 hpi. Upper panels show localization of the fluorescent proteins. Lower panels are integrated time-lapse images. Bars: 10 μm (A and D); 5 μm (B); 25 μm (C, E, and F).