FIG 6.

NB trafficking and ER streaming were affected by dominant negative inhibition of class XI myosins in a similar pattern. NP:YFP (pseudocolored magenta) (A to E) or YFP:HDEL (pseudocolored magenta) (F to J) was coexpressed with CFP:XI-1t (A and F), CFP:XI-2t (B and G), CFP:XI-Jt (C and H), CFP:XI-Kt (D and I), and GUS (E and J). (A′ to J′) Integrated time-lapse images of experiments shown in panels (A to J). (K and L) Quantification of the velocities of NB motility and ER streaming. (K) Percentages of mobile NBs and their average velocities in the presence of the respective myosin tails or GUS. Three movies were analyzed, and the average values with standard deviations are presented. (L) The average and maximum velocities of ER streaming in the presence of myosin tails or GUS. Six movies were analyzed, and the average values and standard deviations are presented. Asterisks indicate significant differences from a GUS control (P < 0.01). (M) A coimmunoprecipitation experiment with NP and class XI myosins. The four CFP fusions with myosin tails or NP:YFP were coexpressed with NP:3myc. The inputs and precipitants were analyzed by immunoblotting using anti-myc and anti-GFP antibodies. Bars, 10 μm.