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Analysis of NP RNA-binding activity. (A) Electron mobility shift assay. The indicated concentrations of MBP:NP and MBP were incubated with the 32P-labeled 5′-terminal region of RNA5 from FMV as a probe. Positions of free and bound RNAs are indicated. (B) Competitor assay. MBP:NP and the FMV probe were incubated with the unlabeled 3′-terminal region of TMV genomic RNA at the indicated molar ratios.
